See SpectraMax® Microplate Reader User Manual Download Page. 0´ 104 6. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. edu) to be added as a trained user in iLab. 58% for the detection module and 2. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. SeeSpectraMax MiniMaxImagingCytometeronpage85. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 313 reference gain check fail. Plate chamber design of the SpectraMax category 1 microplate readers. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Actual shake speed is based on the microplate format. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. 蛍光 発光 ELISA. 02, 0. Figure 4. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Budget-friendly multi-mode microplate reader . 200-800 nm using Spectra Max i3X microplate reader. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. 7. Table 1. Register to download your free eBook today. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Find regional support. Check software compatibility with Windows 10 and locate your software installer. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. User-upgradeable detection modules allow you to expand the capabilities of your microp. Prepare your i3/i3x instrument for shipment. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. The size and fluorescence intensity were tested for 48 h. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Contact Us Phone: +1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Read height should be optimized for . Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. *On the SpectraMax i3x reader only. 2 only) SpectraMax M-series Microplate readers. 04, 0. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. *On the SpectraMax i3x reader only. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. 7 mm for all shake intensities. Reporter Assay. The corrected absorbance is within 5% of the value. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 2 (Molecular Devices, San Jose, CA). The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 99 to $1999. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. Wokingham, Berkshire RG41 5TS United Kingdom. Promega. Example: [Drawer]. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Explore a wealth of applications with the future-ready SpectraMax i3x. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Prepare your iD series instrument for shipment. ZERO BIAS - scores, article reviews, protocol conditions and more. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 5 decades, with sensitive detection of cells. To detect physical stability, Pdots were treated with water and incubated with plants. ATP (“flash” luminescence using . SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Order Now. . This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. See Unpacking the SpectraMax Paradigm Instrument on page 1. 제품. Recommended settings for SpectraMax iD5 and i3x readers. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 흡광 (Absorbance) 리더기. Assay setup for a 384-well low-volume plate. User-upgradeable detection modules allow you to expand the capabilities. Art-science interactive light show/animation. Multimode Reader Comparison Chart. Supplier: Molecular Devices. 蛍光 発光 ELISA. The SpectraMax i3x reader provides the. SpectraMax i3x Multi-Mode Microplate Reader System. com Check our website for a current listing of worldwide distributors. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Example: [Drawer]. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. Learn more about its features, specifications, and applications in this user guide. We would like to show you a description here but the site won’t allow us. ATP standard curves read on SpectraMax microplate readers. Generate more data points without the need to dilute. It allows you to explore cellular pathways and protein activation and expression in one system. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. It allows you to explore cellular pathways and protein activation and expression in one system. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Molecular Devicesの全商品を見る. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. ZERO BIAS - scores, article reviews, protocol conditions and more. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax i3x Multi-Mode Detection Platform. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. each new lot of plates, to ensure that the center of each well is read. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. Modularity does provide some measure of forward vision into upcoming or future experimental needs. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. Unlock your i3/i3x instrument after shipment. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. In this product video, see how the SpectraMax M5 quickly reads samples. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. 2C). Find regional support. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Supplier: Molecular Devices. eu@moldev. . The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. Increased fluorescence performance. com. *On the SpectraMax i3x reader only. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Features. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). Data Analysis. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Cet appareil ISM est confomre à la norme NMB-001 du Canada. o For example: Ex: 485/Em 525/Cutoff 515 4. 0001 0. However, no piece of equipment is immune to breaking down. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. TUNE Tunable Wavelength Detection Cartridge. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. It allows you to explore cellular pathways and protein activation and expression in one system. , Fisher Scienti fi c, cat. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 22 μ m fi ltration unit (Sigma, cat. 2. The SpectraMax i2X is used for cell migration, cell. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Description 1. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. SpectraMax Microplate Readers. Figure 1. each new assay. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. 価格、見積もり、お近くの販売店を. SpectraMax i3x. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. 99. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax i3, i3x, and iD5 readers. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. iD5. Explore a wealth of applications with the future-ready SpectraMax i3x. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. Unlock your i3/i3x instrument after shipment. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The device can be upgraded to include capabilities for Western blotting, imaging and injection. 2. The SpectraMax ® i3x reader provides the. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Average 98 stars, based on 1 article reviews. The information below applies to a standard 96 well plate, set to a linear shake mode. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. china@moldev. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. Description 1. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Examples: fiPress carefully to engage,fl fiDo not press down. , 2014; Xiong et al. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. with best-in-class data analysis software • Three modes of detection for . Multi-Mode Reader. ). SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. More Product Information. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. i3. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. Table 1. Italic and boldface type are used for emphasis. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Introduction. 13. 303 unable to cal dark current. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Remove the transport bracket and store the retaining screws in the bag that held the 3. Features. Prepare your i3/i3x instrument for shipment. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 4. Detection cartridge modularity allows for easy addition of new read modes within minutes without. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 1. Examples: fiPress carefully to engage,fl fiDo not press down. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Note: Additional readers with similar performance are listed. 315 can't find zero order. 0. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Manufacturers try to design their instruments so they can be. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 314 low lamp level warning. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. ZERO BIAS - scores, article reviews, protocol conditions and more. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Amplitude : 1. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Find regional support. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Promega. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. 3) At the bottom of the strip, click on "Show. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. Fluorescence Microplate Reader Comparison Chart. applications, including dual luciferase and ATP assays. 4 μM), prepared as described in section 2. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 모든 제품. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Danaher Inc spectramax i3x. 4. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. Lower limits of detection for ATP determined using the ATPlite . EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. It features a modular design that allows users to add or change detection cartridges according to their needs. The CellTiter-Glo assay from Promega uses luciferase. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Shake intensity. 306 plate air cal fail,low light. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. GFP expression imaged on the cytometer. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. ) wide and 65 cm (25. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. Nuclear Receptor. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Bioz Stars score: 98/100, based on 1 PubMed citations. Molecular Devices are known for both the reliability and the wide uses of their products. Home > Search Results > Danaher Inc > spectramax i3x. Table 1. Also refer to this Multimode Reader Comparison Chart. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The plate was covered and incubated for one hour at room temperature. MTT was purchased from Sigma-Aldrich (St. Replicate values were averaged and imported into Design-Expert 8. Instrument. Microplate Readers SpectraMax. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. 8 Antibodies and. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. SpectraMax i3x Multi-Mode Detection Platform User Guide. With optimized reagents, option to operate the readers. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Time-. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Ferguson, Ph. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. The treatments were. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 7°C (gradient heating design). The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devicesの全商品を見る. The SoftMax Pro Software provides integrated instrument control. 5 µL anti-cAMP-Cryptate. 3: The cuvette chamber. , 2021).